Determining the genetic diversity of silkworm lines in Turkey
نویسندگان
چکیده
Studies involving molecular genetic characterization of silkworms are quite limited. Therefore, with this study, pure and hybrid silkworm lines raised in Turkey was characterized for the first time by microsatellite DNA markers. Samples were collected from 7 different lines, which obtained Provincial Directorate Agriculture Forestry Bursa ones Silkworm Cocoon Association (Kozabirlik). samples extracted eggs. Genotyping done combining a total 16 markers single multiplex. Two hundred eighty-one alleles observed loci. The highest allele number T01CTA07R locus (30), while effective (17.61) polymorphic information content (0.94) FL0612 locus. expected heterozygosity values (He) range 0.72 (K02) to 0.95 (FL0612). lowest (Ho) ranged between 0.52 1.00. When all considered population, only three sixteen loci HWE. As result successful genotyping performed combined multiplex using 3 fluorescent dyes. high polymorphism used showed that these can be effectively identification breeds or lines. This study is fill gaps related literature as well contribute conservation local resources.
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ژورنال
عنوان ژورنال: Turkish Journal of Veterinary & Animal Sciences
سال: 2022
ISSN: ['1300-0128', '1303-6181']
DOI: https://doi.org/10.55730/1300-0128.4213